黄瓜绿斑驳花叶病毒(CGMMV)可借助种子远距离传播，对葫芦科植物的生长和发展造成严重威胁。为有效防控黄瓜绿斑驳花叶病毒(CGMMV)在南瓜上的侵染，在保证南瓜种子发芽及子叶生长不受影响的同时能够有效降低黄瓜绿斑驳花叶病毒(CGMMV)的活性的前提下，寻找南瓜种子干热处理的最适温度和时间条件。我们对南瓜新种子和旧种子进行干热处理，比较了处理温度、持续时间及处理后保存时间对南瓜种子发芽率的影响，并采用实时荧光定量RT-PCR检测病毒的表达量。试验结果表明，处理温度和持续时间及其互作效应都对南瓜种子的发芽率有显著或极显著影响。恒温干热法处理南瓜新种子，处理时间为72 h，处理温度为80 ℃和86 ℃时，种子发芽率不会显著降低，且CGMMV全部灭活。保存1 a的南瓜旧种子，用低于80 ℃的温度处理72 h，发芽率没有显著变化，但当处理温度为86 ℃时，种子发芽率显著降低。变温干热法处理南瓜旧种子，由于累计处理时间长达7 d，当变温中最高温度达到72 ℃以上时，将显著影响南瓜种子的发芽。由此可见，干热处理种子是防治南瓜 CGMMV病毒的有效方法之一。

Cucumber green mottled mosaic virus(CGMMV) can spread over long distances through seeds, posing a serious threat to the growth and development of cucurbitaceae plants. In order to effectively prevent and control the infection of the virus on pumpkins, the optimal temperature and time conditions were searched for dryheat treatment which would not only ensure the germination and cotyledon growth of pumpkin seeds but also reducethe activity of CGMMV. The effects of treatment temperature, duration, and storage time after treatment on the germination rate of pumpkin seeds were compared with new and old pumpkin seeds, and real-time fluorescence quantitative RT-PCR was used to detect the expression level of the virus. The results showed that the treatment temperature, duration, and their interaction effects had a significant and extremely significant impact on the germination rate of pumpkin seeds. When new pumpkin seeds were treated with thermostatic dry heat treatment for 72 hours at 80 ℃ or 86 ℃, the germination rate of the seeds did not significantly decrease, and all CGMMV were inactivated. When the old pumpkin seeds stored for one year were treated at the temperature below 80 ℃ for 72 hours, the germination rate did not show significantly changes. However, when the treatment temperature was 86 ℃, the germination rate of old seeds was significantly decreased. When the alternate temperature dryheat method was used to treat old pumpkin seeds, it would significantly affect the germination of pumpkin seeds due to the cumulative treatment time up to 7 days while the highest temperature among alternate temperature reached above 72 ℃. The experimental results indicated that dryheat treatment of seeds was one of the effective methods for controlling pumpkin CGMMV virus.

S642.1

酒泉市科技支撑计划A类(2022CA1009)。