[关键词]
[摘要]
为了综合评价胡麻资源苗期耐盐性,筛选出合理的胡麻苗期耐盐性鉴定指标,为胡麻耐盐育种和栽培提供基础理论依据,采用300 mmol/L的NaCl溶液对10份胡麻资源进行苗期胁迫处理,分析胁迫对幼苗存活率、株高、地下部干重、地上部干重、生长速率、丙二醛、脯氨酸、叶绿素、超氧化物歧化酶和过氧化物酶等10项指标的影响,结合相关性、主成分、聚类和回归分析方法进行综合评价。结果表明,轮选1号、R40、DYMS和陇亚10号具有相对较强耐盐性。筛选出生长速率和过氧化物酶2个鉴定指标为胡麻苗期耐盐能力鉴定的关键指标,以这2个筛选指标计算出的耐盐性预测值与综合评价计算出的综合Z值之间极显著相关(R2=0.982 2**),表明该鉴定结果具有较高的准确性。将10份胡麻资源的苗期与萌发期耐盐性鉴定结果对比发现,胡麻萌发期与苗期的耐盐性具有不完全的一致性。
[Key word]
[Abstract]
In order to make a comprehensive evaluation on the salinity tolerance and to screen out reasonable salt-tolerance parameters at linseed seedling stage for salt-tolerance breeding and cultivation, in this study, the seedling of 10 linseed germplasm resources were treated with 300 mmol/L NaCl, survival rate, seedling height, root dry weight, shoot dry weight, growth rate, contents of malonaldehyde, proline, chlorophyll, superoxide dismutase and peroxidase were analyzed to study the effect of salt stress on the change of those parameters. Methods of correlation analysis, factor and principal components analysis, clustering analysis and stepwise regression analysis were combined to evaluate salinity tolerance comprehensivly. The results showed that among 10 linseed materials, Lunxuan 1, R40, DYMS and Longya 10 were relativly strong in salt tolerance. Growth rate and content of peroxidase were screened out to be the key parameters for salt-tolerance identification of linseed resources at seedling. The predictive values of 2 key parameters showed a significant correlation with the comprehensive Z values (R2=0.982 2**), which indicated that the results were of high accuracy. By comparing the results of salt tolerance at seedling and germination stage of 10 linseed resources, it was found the salt tolerance of linseed was not completely consistent at germination stage and seedling stage.
[中图分类号]
S565.9
[基金项目]
国家自然科学基金项目(31560347);国家现代农业产业技术体系(CARS-14-2-23);甘肃省知识产权计划项目(21ZSCQ026);甘肃省农业科学院科研条件建设与成果转化重点研发计划项目(2021GAAS18);兰州市人才创新创业项目(2021- RC-64);兰州市引进国外智力成果示范推广基地;兰州市产学研合作基地;兰州市安宁区食药同源资源保护与利用(2022-JB-10)。