[关键词]
[摘要]
将拟南芥AtASR基因核苷酸序列作为查询序列,在NCBI数据库中进行BLAST比对,得到马铃薯StASR基因(GenBank登录号:XM_006359742)的序列。通过生物信息学分析发现,该基因位于4号染色体上,基因长度为654 bp,编码108个氨基酸残基,其编码的蛋白质具有典型的ABA/WDS保守结构域。启动子区域分析后发现,马铃薯StASR基因含有水分响应元件MYCATERD1、冷胁迫响应元件DRECRTCOREAT和盐胁迫响应元件GT1GMSCAM4等多种与非生物胁迫响应相关元件以及马铃薯生长发育相关顺式作用元件,对其进行蛋白质互作网络的分析发现与低温、水分胁迫等基因互作。用PCR技术成功的克隆到马铃薯StASR基因,其结果可以为深入研究马铃薯StASR基因的功能提供基础。
[Key word]
[Abstract]
In this experiment, the sequence of the potato StASR gene(Genbank Accession number:XM_006359742) was obtained by BLAST comparison in NCBI database using the nucleotide sequence of the Arabidopsis AtASR gene as the query sequence. Bioinformatics analysis revealed that the gene was located on chromosome 4 with a length of 654 bp and encodes 108 amino acid residues, and the encoded protein has a typical ABA/WDS conserved domain. After analyzing the promoter region, it was found that the potato StASR gene contains water response element MYCATERD1, cold stress response element DRECRTCOREAT, salt stress response element GT1GMSCAM4 and other abiotic stress response related elements as well as potato growth and development related cis-acting elements. The analysis of protein interaction network found that it interacted with genes such as low temperature and water stress. In addition, the potato StASR gene was successfully cloned by PCR technology, and the results can provide a basis study of the function of the potato StASR gene.
[中图分类号]
Q781;S532
[基金项目]
国家自然科学基金资助项目(31760410);甘肃省种业攻关项目(GZGG-2021-4);现代丝路寒旱农业马铃薯产业发展项目(GNKJ-2020-1);甘肃省农业科学院生物育种专项(2020GAAS10)。