利用生物信息学数据库及软件，对绵羊羟色胺受体4基因(hydroxytryptamine receptor 4，HTR4)进行生物信息学分析，以初步了解其结构并进行功能预测。结果表明，绵羊HTR4基因所含最大长度的开放阅读框为1 317 bp，编码438个氨基酸残基。HTR4基因编码的蛋白分子质量为49 437.09 KDa，理论等电点(pI)为8.32。亚细胞定位结果表明其编码产物主要定位于质膜(60.9%)，且不属于分泌蛋白。HTR4蛋白不存在信号肽序列，存在7段跨膜结构且无低复杂性段区域，该蛋白的二级结构以α螺旋为主，且三级结构主要由α折叠缠绕形成。

The bioinformatics analysis of sheep HTR4 gene was carried out by using bioinformatics database and software， and its structure was preliminarily understood and predicted. The results showed that sheep HTR4 gene contained an open reading frame of 1 317 bp， encoding 438 amino acid residues. The molecular mass of HTR4 protein was 49 437.09 KDa， and the theoretical isoelectric point pI was 8.32. The subcells were mainly located in plasma membrane and did not belong to secretory protein(60.9%). There are no signal peptide sequences. There are seven segments of transmembrane structure and no segments of low complexity region. The secondary structure is mainly α-helix， and the tertiary structure is mainly formed by α-helix， winding and folding.

S826

甘肃农业大学学生科研训练计划项目(202004030)。