利用生物基因组学数据库，对牦牛金属硫蛋白3基因进行生物信息学分析，从而预测 MT-3 基因编码产物的理化性质以及功能结构域，并构建MT-3同源基因的系统进化树。结果表明，牦牛MT-3 基因含有1 个 513 bp 的ORF，编码 170 个氨基酸。MT-3蛋白分子质量约为17 556.37 Da，理论等电点为 5.68。MT-3 编码产物的二级结构主要以β折叠和无规则卷曲为主，推测是非酶类蛋白质。MT-3具有神经生长抑制活性，通过清除自由基和 NO ，从而起到保护脑细胞的作用。

This study aimed to study the bio-information of Wild yak MT-3 gene. The physicochemical characteristics， structures and functions of ovine MT-3 are predicted with software tools and database. Meanwhile， the phylogenetic tree of MT-3 and related proteins is constructed， The results show that the ORF of MT-3 is 513 bp which encoded 170 amino acids. The Wild yak protein of MT-3 is 17 556.37 Da in molecular weight， isoelectric point is 5.68. The secondary structure of MT-3 shows that it is mainly constituted of β-pleated sheet and coils， the encoding production conjecturally belongs to non-enzyme protein. MT-3 has an inhibitory activity on nerve growth， by scavenging free radicals and NO， it plays a role in protecting brain cells.

S826；Q57

甘肃省农牧厅生物技术子课题(GNSW-2011-27)。