对基因工程菌JZ2316发酵的接种量、pH及诱导剂IPTG添加时间等条件进行了研究。结果表明，该菌株的最佳发酵条件为:培养基组成为葡萄糖0.500 0 g/L、磷酸二氢钾0.667 0 g/L、一水柠檬酸0.355 0 g/L、七水硫酸镁0.250 0 g/L、二水氯化钙0.002 5 g/L、消泡剂0.025 0 g/L、微量元素母液(包括七水硫酸铁5.00 g/L、硼酸0.10 g/L、六水氯化钴0.10 g/L、一水硫酸锰0.33 g/L、七水硫酸锌3.80 g/L、二水钼酸钠0.10 g/L、硫酸钴0.10 g/L)0.010 0 g/L，流加糖为60%葡萄糖(质量体积比)，pH为6.9，接种量为10%，发酵OD600 nm达到28添加诱导剂。

The genetically engineered bacterium JZ2316 was one glucosamine fermentation producing strain. The culture conditions such as inoculum size， pH and addition time of inducer were studied to confirm the optimal fermentation conditions. The results showed that the optimal fermentation medium was glucose 0.500 0 g/L， KH2PO4 0.667 0 g/L， C6H8O7·H2O 0.355 0 g/L， MgSO4·7H2O 0.250 0 g/L， CaCl2·2H2O 0.002 5 g/L， defoamer 0.025 0 g/L， microelements mother solution 0.010 0 g/L. The optimum fermentation conditions of glucosamine were 60%(m/v) amount of glucose， pH 6.9， adding inducer when the absorbance at 600 nm reached to 28.

TQ929