以冬油菜陇油6号叶片为材料，利用RT-PCR 法克隆到油菜COR基因编码区序列，全长390 bp。将其与大肠杆菌表达载体pET-30a连接，构建原核表达载体pET-30a-COR，并转化大肠杆菌BL21，经IPTG诱导表达后，SDS-PAGE检测结果表明该基因表达了1个约14.3 kD的蛋白，为进一步研究目的蛋白的结构和功能提供了实验基础。

A full-length of COR gene is cloned by RT-PCR from Longyou 6 (Brassica campestris L.)， the gene encoding area of COR is 390 bp. It is connected with the E. coli expression vector pET-30a. A recombinant prokaryotic expression vector pET30a-COR is constructed and transformed into E.Coli BL21， after IPTG induction， SDS-PAGE showed that the gene express an approximately 14.3 kD protein. For the further study on the structure and function of target protein provides an experimental basis.

Q753；S565.4

国家自然科学基金(31460099、 31160089)；甘肃省自然科学基金(1208RJZA268)