[关键词]
[摘要]
为研究豌豆白粉病抗病性与各生理指标间的关系,选取4种抗性不同的豌豆品种为试材,测定各品种间叶绿素和可溶性糖含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性变化。结果表明,不同抗性品种中叶绿素含量、POD、PPO活性和PAL活性随豌豆白粉病抗性减弱而降低;CAT活性随豌豆白粉病抗性减弱而升高;SOD活性与豌豆白粉病抗性没有明显变化规律。方差分析结果显示,不同抗感病品种间叶绿素含量、PPO活性、PAL活性和CAT活性差异极显著(P<0.01),因此可以用叶绿素含量、PPO活性、PAL活性和CAT活性来反映对豌豆白粉病抗性的强弱。
[Key word]
[Abstract]
It is studied the relations between pea powdery mildew resistance and physiological indicators. Selected 4 kinds of pea with different resistance to pea powdery mildew. The content of soluble sugar and chloroplast changes in activity of specific enzymes such as SOD, CAT, POD, PPO and PAL are detected. The result shows that the content of chloroplast and the activity of POD, PPO and PAL are decreased as the resistance weakened, the activity of CAT was increased as the resistance weakened, while there is no significant correlation in content of the activity of SOD.The result of variance analysis illustrated the significant difference in the content of chloroplast and the activity of PPO, PAL and CAT between different cultivars(P<0.01). So it is confirmed that the content of chloroplast and the activity of PAL and CAT could used as indicator for resistance to pea powdery mildow.
[中图分类号]
S642.2
[基金项目]
现代农业食用豆产业技术体系(CARS-09);国家自然科学基金(31160304);甘肃省青年科技基金计划(145RJYA303); 甘肃省农业科学院中青年基金项目(2014GAAS18)资助